Feeding

Experimental protocol

Detailed procedures for preparing and recording images of Caenorhabditis elegans phenotypes can be found at the following links:

High-throughput image-based drug screening of Caenorhabditis elegans movement, development, and viability
Pharmacological Profiling of a Brugia malayi Muscarinic Acetylcholine Receptor as a Putative Antiparasitic Target (feeding assay is described in the methods)

Configuration of the GUI

The Feeding analysis is implemented via a CellProfiler pipeline that uses a custom Worm Toolbox model. In Pipeline Selection, choose CellProfiler and select the Feeding pipeline.

Custom worm models

Performance of the pipeline will vary based on the magnification used to take the images. If you are interested in training your own Worm Toolbox model, please reach out to the wrmXpress developers.

Expected input

Feeding data may be analyzed in the form of individual TIF images per frame (i.e., the TimePoint structure utilized by ImageXpress). See the Data Organization page for more details. In the case of individual TIF images per frame, the directory structure should look like this:

Feeding file structure

All experiments should include three wavelengths and single site.

Validated species and stages

Caenorhabditis elegans young adults

Example plates

20210823-p01-KJG_795: Caenorhabditis elegans young adults

Expected output

A CSV file with at many columns of data generated by with CellProfiler's Worm Toolbox. If using Metadata, there will be an additional column for each provided metadata data frame.